Journal: Brazilian Journal of Microbiology

Article Title: An investigation of virulence factors of Legionella pneumophila environmental isolates

doi: 10.1016/j.bjm.2017.03.012

Figure Lengend Snippet: The intracellular growth of bacteria in different host cell. 1: the I2 in PBMCs; 2: the I5 in PMs; 3: the I5 in A . castellanii. A, B, C: penetration, intracellular growth at the 24th and 72nd hours in light microscopy; D, E, F: penetration, intracellular growth at the 24th and 72nd hours in epifluorescence microscopy. Sacale Bar = 10 μm in Picture 1; 25 μm in Picture 2; 50 μm in Picture 3 (100×).

Article Snippet: The in vitro infection capabilities of L. pneumophila isolates were studied in various hosts such as: (i) peripheral blood mononuclear cells (PBMCs) obtained from blood samples collected from volunteers, (ii) peritoneal macrophages (PMs) obtained from BALB/c female mice by induction with thioglycolate (TGC) medium (the procedure was approved by the Istanbul University animal experimentation local ethics commission; decision number: 2011/87), and (iii) A. castellanii ATCC 30234 trophozoites.

Techniques: Bacteria, Light Microscopy, Epifluorescence Microscopy

Journal: Brazilian Journal of Microbiology

Article Title: An investigation of virulence factors of Legionella pneumophila environmental isolates

doi: 10.1016/j.bjm.2017.03.012

Figure Lengend Snippet: Detection of the adherence, entry (0 h) and intracellular growing (24 h, 48 h and 72 h) capacity of L. pneumophila on PBMCs (A), PMs (B) and A. castellanii trophozoites (C).

Article Snippet: The in vitro infection capabilities of L. pneumophila isolates were studied in various hosts such as: (i) peripheral blood mononuclear cells (PBMCs) obtained from blood samples collected from volunteers, (ii) peritoneal macrophages (PMs) obtained from BALB/c female mice by induction with thioglycolate (TGC) medium (the procedure was approved by the Istanbul University animal experimentation local ethics commission; decision number: 2011/87), and (iii) A. castellanii ATCC 30234 trophozoites.

Techniques:

Journal: Frontiers in Immunology

Article Title: C2 IgM Natural Antibody Enhances Inflammation and Its Use in the Recombinant Single Chain Antibody-Fused Complement Inhibitor C2-Crry to Target Therapeutics to Joints Attenuates Arthritis in Mice

doi: 10.3389/fimmu.2020.575154

Figure Lengend Snippet: In vitro study showing binding of IRDye 800 labeled C2-Crry (Green color) to serum starved FLS undergoing apoptosis. FLS were serum starved in 24-well tissue culture plates for 72 h followed by the addition of IRDye 800 labeled C2-Crry. (A) FLS control bright field showing little to no apoptosis. (B) Apoptotic FLS bound to labeled C2-Crry (Green color; red arrow) in 24-well plate. (C) Caspase 3/7 staining (2 mM) staining used for negative control FLS on the glass slide showing minimum background green color staining. (D) Caspase 3/7 staining used to show serum starved apoptotic FLS for 6 h on a glass slide with cover slip. Apoptotic cells and apoptotic bodies appeared green under UV light as shown by red arrows. Scanning/and or imaging using with Zeiss Axio Observer 5 epifluorescent microscope equipped with X-Cite 200 DC light source and Axiocam 506 monochromatic camera. Near infrared Fluorescence was imaged using Cy7 filter set (Chroma Corporation, McHenry, IL, United States). One representative experiment of C2-Crry binding to apoptotic FLS images is shown. No scale was added when the images were taken. But 40x magnification objective was used to take these pictures for images (A,C) and 10x objective was used for images (C,D) .

Article Snippet: Binding of IRDye 800 labeled C2-Crry to apoptotic FLS was observed using a Zeiss Axio Observer 5 epifluorescent microscope ( ).

Techniques: In Vitro, Binding Assay, Labeling, Control, Staining, Negative Control, Imaging, Microscopy, Fluorescence